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KMID : 0848120080330020051
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International Journal of Oral Biology
2008 Volume.33 No. 2 p.51 ~ p.58
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The effect of UNCL inactivation on the expression of mechanical stress related genes in cultured human PDL fibroblasts
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Choi Yong-Seok
Jang Hyun-Sun
Lee Dong-Seol
Kim Heung-Joong
Park Jong-Tae
Bae Hyun-Sook
Park Joo-Cheol
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Abstract
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A mutation of UNCL, an inner nuclear membrane RNAbinding protein, has been found to eliminate mechanotransduction in Drosophila. UNCL is expressed in human periodontal tissue including in periodontal ligament (PDL) fibroblasts. However, it is unclear how a mechanical stimulus is translated into cellular responses in PDL fibroblasts. The aim of this study was to evaluate the effect of UNCl on mechanical stress related genes in PDL fibroblasts in response to mechanical stress. The mRNA of TGF-β, COX-2, and MMP-2 was up-regulated after UNCL inactivation in PDL fibroblasts under the compression force. Under the tensile force, inactivation of UNCL decreased the expression of Biglycan, RANKL, MMP-2, and TIMP-2 mRNAs while it increased the expression of TIMP-1. p38-MAPK was expressed in PDL fibroblasts under compression forces whereas phospho-ERK1/2, p65-NFkB, and c-fos were expressed under tension forces. The expression and phosphorylation of the mechanical stress related genes, kinases, and transcription factors were changed according to the types of stress. Furthermore, most of them were regulated by the inactivation of UNCL. This suggests that UNCL is involved in the regulation of mechanical stress related genes through the signaling pathway in PDL fibroblasts.
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KEYWORD
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UNCL, Mechanical stress, PDL fibroblast, Inactivation
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